![]() Some masters can be set to behave like custom widgets and this allows them to removed from the master list if necessary. ‘The ‘Do Not Generate Masters Set as Custom Widgets’ does exactly that, funnily enough. If ‘All Pages’ is set on that tab by definition all masters will also all be displayed. ‘Only Generate Masters Used on Generated Pages’ only includes masters that are are used in the selected pages from the ‘Pages’ tab. The first option – ‘Only List Generated Masters’ is useful if there are masters in the project that are not being generated because of one of the other options in this screen (explained below, but useful for the iterative / modular spec approach). The interesting set (the other options are essentially the same as document above for ‘Pages’) are around which masters to generate and whether to generate them once or as they used per page. ![]() In your specification, you have the option to print out all or a select few masters as you did with pages, change section and heading titles. It’s a concept that is used in traditional manual specifications but is harder to manage – primarily because it relies on manual processes to apply changes to the relevant areas. Make a change for one master and its applied across them all. Masters in Axure are used to define common elements that appear across the system and allow a centralised governance. When generated in the specification, the following section of the spec is effected by the ‘Pages’ configuration options. ![]() This way you can flesh out the annotations / notes for the relevant pages and leave out those that are not finished. This is particularly useful when developing in an iterative approach where the specification is built a functional area or module at a time. Then the next section allows you to select what pages will be included in the generated spec. Starting with the first options, there is an option to include the pages section and give it a title and directly beneath that an option to specify whether to include a sitemap and change it’s title. So the first tab, ‘Pages’ allows you to select which of the pages within your project you wish to include in the generated specification. Pages – a blessing for BA’s working within an iterative project I want to explore the available options on each tab of the configuration window and show the effect each has on the generated spec. Lets assume you already have a project to work from that will generate a useful specification and press ‘F6’ or the ‘Generate Specification’ on the toolbar. This really only touched on one tab of the specification generation configuration and the idea of this post is to go over the remaining options and explore their usage. In the first part of this post, I went through the process of creating and refining a word template to suit a generated Axure specification.
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In cultures of primary human renal tubular epithelial cells (RPTECs) subjected to anoxia-reoxygenation, inhibition of the Krebs cycle at the level of malate dehydrogenase-2 (MDH-2) decreases hypoxia-inducible factor-1α and oxidative stress and protects from apoptotic or ferroptotic cell death. Reactive oxygen species (ROS) production causes cell death or senescence. Ischemia-reperfusion injury is the leading cause of acute kidney injury. Additionally, milk lactose concentrations suggest that MAP reduces the availability of lactose derivatives. The targeted metabolites were suggestive of wider changes in the bioenergetic metabolism that appear to be an acceleration of the effects of progressing lactation in healthy cows. Pearson's correlation analysis indicated relationships between milk lactose concentrations in mid-lactation and 6 metabolites that were tentatively linked to MAP-infection status. Pathway enrichment analysis suggested that MAP affected the malate-aspartate shuffle during early lactation. Examining each lactation stage separately for changes associated to MAP-infection status identified 45 metabolites, 33 in early lactation and 12 in mid-lactation, but only 6 metabolites were targeted in both stages of lactation. Metabolite fingerprinting assessments using partial least squares discriminate analyses (PLS-DA) indicated that lactation stage was a larger source of variation than MAP status. The milk metabolome was assessed using flow infusion electrospray high-resolution mass spectrometry (FIE-HRMS) for sensitive, non-targeted metabolite fingerprinting. The study used biobanked milk samples which were collected 73.4 ± 3.79 (early lactation) and 143 ± 3.79 (mean ± SE) (mid-lactation) days post-calving from 5 MAP-infected and 5 control multiparous cows. Herein, we examine the milk metabolomic profiles of naturally MAP-infected Holstein-Friesian cows. Infected cows begin shedding MAP within the asymptomatic, subclinical stage of infection before clinical signs, such as weight loss, diarrhoea and reduced milk yields develop within the clinical stages of disease. Mycobacterium avium subspecies paratuberculosis (MAP) is the causative organism of Johne's Disease, a chronic intestinal infection of ruminants. This illustrates the vitality of ongoing MAS research. The year 2019 saw the discovery of two new inborn errors in the MAS, deficiencies in malate dehydrogenase 1 and in aspartate transaminase 2 (GOT2). Most recently, the focus has been on the role of the MAS in tumors, on cells with defects in mitochondria and on inborn errors in the MAS. The MAS is still a very active field of research. This makes the MAS in practice uni-directional toward oxidation of cytosolic NADH, and explains why the free NADH/NAD ratio is much higher in the mitochondria than in the cytosol. Only in the 1970s, LaNoue and coworkers discovered that the efflux of aspartate from mitochondria, an essential step in the MAS, is dependent on the proton-motive force generated by the respiratory chain: for every aspartate effluxed, mitochondria take up one glutamate and one proton. The MAS was soon adopted in the field as a major pathway for NADH oxidation in mammalian tissues, such as liver and heart, even though the energetics of the MAS remained a mystery. ![]() The MAS was initially proposed as a route for the oxidation of cytosolic NADH by the mitochondria in Ehrlich ascites cell tumor lacking other routes, and to explain the need for a mitochondrial aspartate aminotransferase (glutamate oxaloacetate transaminase 2 ). ![]() This article presents a personal and critical review of the history of the malate-aspartate shuttle (MAS), starting in 1962 and ending in 2020. |
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